Characterization of ISApl1

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• 作者：Jinlin Liu ; Chen Tan ; Jinquan Li ; Huiwen Chen ; Peng Xu ; Qigai He ; Weicheng Bei ; Huanchun Chen
• 关键词：Actinobacillus pleuropneumoniae ; IS1070 ; pgaC ; Biofilm
• 刊名：Veterinary Microbiology
• 出版年：2008
• 出版时间：10 December 2008
• 年：2008
• 卷：132
• 期：3-4
• 页码：348-354
• 全文大小：608 K

文摘

An insertion sequence (IS), designated ISApl1, was identified in Actinobacillus pleuropneumoniae. It was 1072 bp in length, and contained a large open reading frame (ORF), which encoded a putative transposase whose sequence was similar to that of transposases of various IS elements of the IS30 family. Another small ORF, a putative antisense repressor of transposase, was located in the opposite direction of transposase. ISApl1 generated a 3-bp duplication of the target DNA and carried 24-bp inverted repeats sequence. ISApl1 was identified in the genome of a biofilm-formation negative A. pleuropneumoniae strain field isolate HB04 and inserted into an A/T rich region of the ORF of pgaC, which encoded the PGA [N-acetyl-d-glucosamine residues in β(1,6) linkage] synthesizing N-glycosyltransferase. The genotype of the pgaC⁻/IS⁺ was not altered after re-isolation from challenged mice, which indicated that this IS element was relatively stable in A. pleuropneumoniae during infection.