Cinnamon polyphenols attenuate the hydrogen peroxide-induced down regulation of S100尾 secretion by regulating sirtuin 1 in C6 rat glioma cells

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• 作者：Bolin Qin^a ; ^b ; ^{Bolin.Qin@ars.usda.gov" class="auth_mail} ; ^{bolin1588@hotmail.com" class="auth_mail} ; Kiran S. Panickar^b ; ^c ; ² ; Richard A. Anderson^b
• 关键词：Polyphenols ; S100尾 ; SIRT1 ; Oxidative stress
• 刊名：Life Sciences
• 出版年：25 April, 2014
• 年：2014
• 卷：102
• 期：1
• 页码：72-79
• 全文大小：1001 K

文摘

Aims

It is well established that the brain is particularly susceptible to oxidative damage due to its high consumption of oxygen. The objective of this study was to investigate the protective effects of a water soluble polyphenol-rich extract of cinnamon and the possible mechanisms, under conditions of oxidative stress-induced by hydrogen peroxide, in rat C6 glioma cells.

Main methods

After 24 h of H₂O₂ incubation, the secretion and intracellular expression of S100尾 were determined by immunoprecitation/immunoblotting and immunofluorescence imaging.

Key findings

Cinnamon polyphenols (CP) counteracted the oxidative effects of H₂O₂ on S100尾 secretion and expression. CP also enhanced the impaired protein levels of sirtuins 1, 2, and 3, which are deacetylases important in cell survival. H₂O₂ also induced the overexpression of the proinflammatory factors, TNF-伪, phospho-NF-魏B p65, as well as of Bcl-xl, Bax and Caspase-3, which are all the members of the Bcl-2 family. CP not only suppressed the expression of these proteins but also attenuated the phosphorylation induced by H₂O₂. CP also upregulated the decreased Bcl-2 protein levels in H₂O₂ treated C6 cells. The effects of CP on H₂O₂-induced downregulation of S100尾 secretion were blocked by SIRT1 siRNA demonstrating that SIRT1 plays a regulatory role in CP-mediated prevention by H₂O₂.

Significance

These data demonstrate that Cinnamon polyphenols may exert neuroprotective effects in glial cells by the regulation of Bcl-2 family members and enhancing SIRT1 expression during oxidative stress.