Inhibition of K562 leukemia angiogenesis and growth by selective Na⁺/H⁺ exchanger inhibitor cariporide through down-regulation of pro-angiogenesis factor VEGF

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• 作者：Wei  ; Gao ; Guoqiang  ; Chang ; Jian  ; Wang ; Weina  ; Jin ; Lihong  ; Wang ; Yani  ; Lin ; Huawen  ; Li ; Li  ; Ma ; Qinghua  ; Li ; Tianxiang  ; Pang  ; ;   ; ^{pang@ihcams.ac.cn}
• 关键词：Na⁺/H⁺ exchanger isoform1 (NHE1) ; Vascular endothelial growth factor (VEGF) ; K562 cells ; Intracellular pH (pHi) ; Angiogenesis ; Leukemia
• 刊名：Leukemia Research
• 出版年：2011
• 出版时间：November 2011
• 年：2011
• 卷：35
• 期：11
• 页码：1506-1511
• 全文大小：899 K

文摘

To investigate the effect of inhibition of Na⁺/H⁺ exchanger isoform1 (NHE1) on K562 leukemia-driven angiogenesis, the selective NHE1 inhibitor cariporide was used. Cariporide treatment of K562 resulted in a decrease in pHi and down-regulation of VEGF secretion. The proliferation, migration and in vitro tube formation of human umbilical vein endothelial cells was decreased in cariporide treated K562 condition medium (CM) while VEGF supplement could partially restore the inhibitory effect. Subcutaneous injection of nude mice with cariporide inhibited K562 tumor growth with a reduction of the density of microvessels compared to the control group.