The effects of fluid shear stress on proliferation and osteogenesis of human periodontal ligament cells

详细信息    查看全文

• 作者：Lisha Zheng^a ; Luoping Chen^a ; Yuchao Chen^a ; Jinpeng Gui^a ; Qing Li^c ; Yan Huang^a ; Meili Liu^a ; Xiaolin Jia^a ; Wei Song^a ; Jing Ji^a ; Xianghui Gong^a ; Ruoshi Shi^d ; Yubo Fan^a ; ^b ; ^{yubofan@buaa.edu.cn" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Human periodontal ligament cells ; Proliferation ; Migration ; Osteogenesis ; Shear stress
• 刊名：Journal of Biomechanics
• 出版年：2016
• 出版时间：29 February 2016
• 年：2016
• 卷：49
• 期：4
• 页码：572-579
• 全文大小：3207 K

文摘

Shear stress is one of the main stress type produced by speech, mastication or tooth movement. The mechano-response of human periodontal ligament (PDL) cells by shear stress and the mechanism are largely unknown. In our study, we investigated the effects of fluid shear stress on proliferation, migration and osteogenic potential of human PDL cells. 6 dyn/cm² of fluid shear stress was produced in a parallel plate flow chamber. Our results demonstrated that fluid shear stress rearranged the orientation of human PDL cells. In addition, fluid shear stress inhibited human PDL cell proliferation and migration, but increased the osteogenic potential and expression of several growth factors and cytokines. Our study suggested that shear stress is involved in homeostasis regulation in human PDL cells. Inhibiting proliferation and migration potentially induce PDL cells to respond to mechanical stimuli in order to undergo osteogenic differentiation.