Nitric oxide is required for hydrogen gas-induced adventitious root formation in cucumber

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• 作者：Yongchao Zhu ; Weibiao Liao ; ^{liaowb@gsau.edu.cn" class="auth_mail" title="E-mail the corresponding author} ; Meng Wang ; Lijuan Niu ; Qingqing Xu ; Xin Jin
• 关键词：Hydrogen-rich water ; Nitric oxide (NO) ; NO synthase (NOS) ; Nitrate reductase (NR) ; NR genes ; Enzyme
• 刊名：Journal of Plant Physiology
• 出版年：2016
• 出版时间：20 May 2016
• 年：2016
• 卷：195
• 期：Complete
• 页码：50-58
• 全文大小：1383 K

文摘

Hydrogen gas (H₂) is involved in plant development and stress responses. Cucumber explants were used to study whether nitric oxide (NO) is involved in H₂-induced adventitious root development. The results revealed that 50% and 100% hydrogen-rich water (HRW) apparently promoted the development of adventitious root in cucumber. While, the responses of HRW-induced adventitious rooting were blocked by a specific NO scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide potassium salt (cPTIO), NO synthase (NOS) enzyme inhibitor N^G-nitro-l-arginine methylester hydrochloride (l-NAME) and nitrate reductase (NR) inhibitor NaN₃. HRW also increased NO content and NOS and NR activity both in a dose- and time-dependent fashion. Moreover, molecular evidence showed that HRW up-regulated NR genes expression in explants. The results indicate the importance of NOS and NR enzymes, which might be responsible for NO production in explants during H₂-induced root organogenesis. Additionally, peroxidase (POD) and indoleacetic acid oxidase (IAAO) activity was significantly decreased in the explants treated with HRW, while HRW treatment significantly increased polyphenol oxidase (PPO) activity. In addition, cPTIO, l-NAME and NaN₃ inhibited the actions of HRW on the activity of these enzymes. Together, NO may be involved in H₂-induced adventitious rooting, and NO may be acting downstream in plant H₂ signaling cascade.