Rice protein hydrolysate attenuates hydrogen peroxide induced apoptosis of myocardiocytes H9c2 through the Bcl-2/Bax pathway

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• 作者：Tao Yang ; He Zhu ; Hui Zhou ; Qinlu Lin ; Wenjian Li ; Junwen Liu
• 关键词：Rice protein hydrolysate ; Myocardiocytes ; Apoptosis ; Bcl-2 ; Bax
• 刊名：Food Research International
• 出版年：2012
• 出版时间：October, 2012
• 年：2012
• 卷：48
• 期：2
• 页码：736-741
• 全文大小：795 K

文摘

Rat myocardiocytes H9c2 were pre-treated with rice protein hydrolysate and then treated with hydrogen peroxide (H₂O₂). The apoptosis rate and caspase-3 activity of H9c2 cells were analyzed by Flow Cytometry and Caspase Activity Detection Assay, respectively; the expressions of B cell lymphoma/leukemia-2 (Bcl-2) and Bcl-2 associated X protein (Bax) were determined by real time-PCR and Western blot. The results showed that apoptosis of H9c2 cells was alleviated in response to the treatment of rice protein hydrolysate, potentially resulting from the up-regulation of Bcl-2 and the down-regulation of Bax. The results indicated that rice protein hydrolysate attenuated the apoptosis of myocardiocytes H9c2 involving in the Bcl-2/Bax pathway during H₂O₂ challenge, which suggest a potential role of rice protein hydrolysate on the healthcare of cardiovascular disease, especially acute myocardial ischemia.