Enhancement of isomerization activity and lactulose production of cellobiose 2-epimerase from Caldicellulosiruptor saccharolyticus
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- 作者：Qiuyun Shena ; b ; c ; Yuzhu Zhangc ; Ruijin Yangb ; yrj@jiangnan.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Siyi Pana ; Juan Dongb ; Yuting Fanb ; Liang Hanb
- 关键词：Lactulose ; Cellobiose 2-epimerase ; Activity improvement ; Random mutagenesis
- 刊名：Food Chemistry
- 出版年：2016
- 出版时间：15 September 2016
- 年：2016
- 卷：207
- 期：Complete
- 页码：60-67
- 全文大小：2421 K
文摘
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A microplate screening procedure was developed for directed evolution of CsCE.
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Mutant G4-C5 was obtained after four rounds of random mutagenesis and screening.
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Mutant G4-C5 has a 2.8-fold increase in activity and a 3.0-fold increase in kcat/Km.
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Yield of lactulose catalyzed by mutant G4-C5 was 76% with no epilactose detected.