Modulation of pro-inflammatory mediators in LPS-stimulated human periodontal ligament cells by chitosan and quaternized chitosan
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- 作者：Qiuxia Ji ; jqx_1@163.com ; Jing Deng ; Xinbo Yu ; Quanchen Xu ; Hong Wu ; Jianfeng Pan
- 关键词：Chitosan ; Quaternized chitosan (HTCC) ; LPS ; Periodontal disease ; Interleukin (IL)-1¦Â ; Tumor necrosis factor alpha (TNF-¦Á)
- 刊名：Carbohydrate Polymers
- 出版年：2013
- 出版时间：30 January, 2013
- 年：2013
- 卷：92
- 期：1
- 页码：824-829
- 全文大小：776 K
文摘
The aim of this study was to evaluate the effects of chitosan and quaternized chitosan (HTCC) modulate IL-1¦Â and TNF-¦Á in LPS-stimulated human periodontal ligament cells (HPDLCs). MTT assay revealed that chitosan stimulated the proliferation of HPDLCs. However, HTCC inhibited the proliferation of HPDLCs at concentrations of 1000 and 100 ¦Ìg/mL more than the control, especially after 5 d (P < 0.001). ELISA assay exhibited that chitosan inhibited the production of IL-1¦Â and TNF-¦Á at 24, 48 and 72 h. IL-1¦Â and TNF-¦Á secreted by HPDLCs with LPS and treated with 1000 ¦Ìg/mL of HTCC significantly increased compared to both the control and the chitosan group (P < 0.001). The bioactive role for bFGF in modulating the responses of HPDLCs cells to LPS via inhibiting IL-1¦Â and TNF-¦Á production was demonstrated. All results were necessary to enhance our understanding of the biomedical properties of chitosan and HTCC for modulation of pro-inflammatory mediators.