Interaction of 17¦Â-estradiol and ketoconazole on endocrine function in goldfish (Carassius auratus)

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• 作者：Zhenhua Yan ; Guanghua Lu ; ^{ghlu@hhu.edu.cn} ; Donghai Wu ; Qiuxia Ye ; Zhengxin Xie
• 关键词：17¦Â-Estradiol ; Carassius auratus ; Endocrine function ; Interaction ; Ketoconazole
• 刊名：Aquatic Toxicology
• 出版年：2013
• 出版时间：15 May, 2013
• 年：2013
• 卷：132-133
• 期：Complete
• 页码：19-25
• 全文大小：530 K

文摘

An understanding of the effects of toxic mixtures of endocrine disrupting chemicals (EDCs) on aquatic organisms is challenging as these organisms are exposed to multiple classes of contaminants in their natural habitat. The aim of the present study was to evaluate the interactions of two classes of EDCs, 17¦Â-estradiol (E₂) and ketoconazole (KTC), on endocrine function in male goldfish (Carassius auratus), including vitellogenesis, metabolic capability and serum steroid synthesis. Changes in vitellogenin (VTG) concentration, liver 7-ethoxyresorufin-O-deethylase (EROD) activity and circulating serum E₂ level were examined. The expression of related genes was also determined using quantitative real-time polymerase chain reaction. Exposure to E₂ caused a significant increase in VTG concentrations which corresponded with the gene expression of VTG and estrogen receptor (ER) in males, which were further elevated after combined exposure to E₂ and KTC, indicative of a synergetic relationship. Exposure to E₂ also resulted in a distinct increase in serum steroid biosynthesis and associated cytochrome P450 (CYP) aromatase expression after 10 days. However, these changes were inhibited by the presence of KTC, which acted as a steroidogenic inhibitor in fish. Moreover, KTC significantly decreased liver EROD activity and increased the related gene expression of CYP1A. However, these KTC-mediated metabolic reactions in goldfish were up-regulated following exposure to KTC in combination with E₂. These findings reveal complex interactions on endocrine functions in male goldfish when exposed to multiple contaminations and may provide a better understanding of the effects of toxic mixtures.