Enhanced silencing and stabilization of siRNA polyplexes by histidine-mediated hydrogen bonds
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- 作者：Szu-Ting Chou ; Kellie Hom ; Daoning Zhang ; Qixin Leng ; Lucas J. Tricoli ; Jason M. Hustedt ; Amy Lee ; Michael J. Shapiro ; Joonil Seog ; Jason D. Kahn ; A. James Mixson
- 关键词：Histidine ; Hydrogen bond ; HK peptide ; siRNA ; Isothermal titration calorimetry ; Nuclear magnetic resonance
- 刊名：Biomaterials
- 出版年：January, 2014
- 年：2014
- 卷：35
- 期：2
- 页码：846-855
- 全文大小：2421 K
文摘
Branched peptides containing histidines and lysines (HK) have been shown to be effective carriers for DNA and siRNA. We anticipate that elucidation of the binding mechanism of HK with siRNA will provide greater insight into the self-assembly and delivery of the HK:siRNA polyplex. Non-covalent bonds between histidine residues and nucleic acids may enhance the stability of siRNA polyplexes. We first compared the polyplex biophysical properties of a branched HK with those of branched asparagine-lysine peptide (NK). Consistent with siRNA silencing experiments, gel electrophoresis demonstrated that the HK siRNA polyplex maintained its integrity with prolonged incubation in serum, whereas siRNA in complex with NK was degraded in a time-dependent manner. Isothermal titration calorimetry of various peptides binding to siRNA at pH 7.3 showed that branched polylysine, interacted with siRNA was initially endothermic, whereas branched HK exhibited an exothermic reaction at initial binding. The exothermic interaction indicates formation of non-ionic bonds between histidines and siRNA; purely electrostatic interaction is entropy-driven and endothermic. To investigate the type of non-ionic bond, we studied the protonation state of imidazole rings of a selectively 15N labeled branched HK by heteronuclear single quantum coherence NMR. The peak of N未1-H tautomers of imidazole shifted downfield (in the direction of deprotonation) by 0.5-1.0聽ppm with addition of siRNA, providing direct evidence that histidines formed hydrogen bonds with siRNA at physiological pH. These results establish that histidine-rich peptides form hydrogen bonds with siRNA, thereby enhancing the stability and biological activity of the polyplex in聽vitro and in聽vivo.