l-Carnitine in rooster semen cryopreservation: Flow cytometric, biochemical and motion findings for frozen-thawed sperm

详细信息    查看全文

• 作者：A. Fattah^a ; ^{amir1356fattah@qodsiau.ac.ir} ; M. Sharafi^b ; ^{m.sharafi@modares.ac.ir} ; R. Masoudi^c ; A. Shahverdi^d ; V. Esmaeili^d ; A. Najafi^e
• 关键词：Fowl ; Sperm freezing ; Mitochondria activity ; Apoptosis
• 刊名：Cryobiology
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：74
• 期：Complete
• 页码：148-153
• 全文大小：445 K
• 卷排序：74

文摘

Rooster semen cryopreservation is not efficient for artificial insemination in breeder flocks. l-Carnitine (LC) has been evaluated for effectiveness in cryopreservation media on the characteristics of rooster sperm after freeze-thawing. Motility characteristics, membrane functionality, abnormal morphology, apoptotic like changes, mitochondria activity and lipid peroxidation of rooster sperms were assessed after freeze-thawing with different concentrations of LC in Beltsville medium. Semen samples were collected from 12 roosters, twice a week, and diluted in the extenders that contained different concentrations of LC. Supplementation of Beltsevile with 1 and 2 mM LC was found to result in higher total motility (68.2± 1.7% and 69.1± 1.7%, respectively), progressive motility (28.4± 1.6%, 29.8± 1.6%), membrane functionality (76.2± 1.9% and 75.9± 1.9%), viability (58.2 ± 1.1%, 59.1 ± 1.1%) and lower significant of lipid peroxidation (2.53 ± 0.08 nmol/ml, 2.49 ± 0.08 nmol/ml) compared to control group containing no LC. Lower motility, progressive motility, and viability were observed in frozen-thawed sperm in extender containing 8 mM LC (35.8± 1.7%, 9.6± 1.2% and 27.1 ± 1.2%, respectively) compared to control. Morphology and mitochondrial activity were not affected by different concentrations of LC. Our results showed that supplementation of Beltsville extender with 1 and 2 mM LC significantly improved the quality of rooster sperm quality after freeze-thawing.