Study on the genotoxicity of HA/ZrO₂ composite particles in vitro

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• 作者：Renfu Quan^a ; ^{quanrenfu8@yahoo.com} ; Yanghua Tang^a ; Zhongming Huang^a ; Jinwei Xu^a ; Xiaochen Wu^b ; Disheng Yang^c
• 关键词：Zirconia ; Hydroxyapatite ; Composite ; Genotoxicity
• 刊名：Materials Science and Engineering: C
• 出版年：2013
• 出版时间：1 April, 2013
• 年：2013
• 卷：33
• 期：3
• 页码：1332-1338
• 全文大小：1446 K

文摘

To evaluate the genotoxicity of the HA/ZrO₂ composite particles by using the micronucleus test (MNT) in vitro. HA/ZrO₂ composite particles prepared by sintering at high temperature and pressure, that used powder of HA and ZrO₂ of different proportions, were compared with pure HA particles and pure ZrO₂ particles. The effect of the composite particles on cell proliferation of rabbit mesenchymal stem cells, and its the genotoxicity to rabbit mesenchymal stem cells were detected by MNT method. The MTT test showed that both pure HA particles and composite particles which contained HA promoted cell proliferation of rabbit mesenchymal stem cells, while pure ZrO₂ particles did not, and there was a significant difference (P < 0.05). The MNT test showed no significant difference between the HA group and the negative control group (P > 0.05), but a significant difference between the HA group and the positive control group (P < 0.05). The difference between the ZrO₂ group and the negative control group was significant (P < 0.01), while the difference between the ZrO₂ group and the positive control group was insignificant (P > 0.05). The genotoxicity of the HA/ZrO₂ composite particle increased with a higher proportion of ZrO₂ and an increase in the concentration of the composite, and the 30 wt. % HA/70 % ZrO₂ composite with 200 ¦Ìg/mL concentration showed significant genotoxicity (P < 0.01).