Multiplex and accurate quantification of acute kidney injury biomarker candidates in urine using Protein Standard Absolute Quantification (PSAQ) and targeted proteomics
There is a need for multiplex and quantitative methods to speed-up the development of acute kidney injury biomarkers. We report the development of a targeted proteomic assay to quantify 4 AKI biomarker candidates in urine samples. Thanks to the use of isotopically-labeled proteins as quantification standards, the assay showed excellent performance. The assay confirmed the relevance of NGAL and L-FABP in AKI diagnosis.