Correlating the structures and activities of the resting oxidized and native intermediate states of a small laccase by paramagnetic NMR
详细信息 查看全文
- 作者：Michael C. Machczynski ; machczym@newpaltz.edu" class="auth_mail" title="E-mail the corresponding author ; Jeffrey T. Babicz Jr.1
- 关键词：Paramagnetic NMR ; Oxidase ; Copper ; Native intermediate ; Exchange coupling ; Laccase
- 刊名：Journal of Inorganic Biochemistry
- 出版年：2016
- 出版时间：June 2016
- 年：2016
- 卷：159
- 期：Complete
- 页码：62-69
- 全文大小：869 K
文摘
- •
The native intermediate state of the small laccase persists for months at 4 °C and pH 7.
- •
An isolated Type-3 copper site produces the 1H NMR spectrum of the resting oxidized state.
- •
The site of three coupled Cu ions produces the 1H NMR spectrum of the native intermediate.
- •
A method for calculating the chemical shifts in an oxidized Cu3 cluster is developed.
- •
The energy of the quartet state supports the model of a hydroxo ligand at the Type-2 site.