Use of green fluorescent protein labeled non-tuberculous mycobacteria to evaluate the activity quaternary ammonium compound disinfectants and antibiotics

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• 作者：Claudia Cortesia^a ; Teresita Bello^b ; Gustavo Lopez^a ; Scott Franzblau^c ; Jacobus de Waard^b ; Howard Takiff^a ; ^{htakiff@gmail.com}
• 关键词：Quaternary Ammonium Compounds (QACs) ; Disinfectants ; NonTuberculous Mycobacteria (NTM) ; Resistance ; Post-surgical infections
• 刊名：Brazilian Journal of Microbiology
• 出版年：2017
• 出版时间：January-March 2017
• 年：2017
• 卷：48
• 期：1
• 页码：151-158
• 全文大小：1250 K
• 卷排序：48

文摘

Although infections with NonTuberculous Mycobacteria have become less common in AIDS patients, they are important opportunistic infections after surgical procedures, likely because they are ubiquitous and not efficiently killed by many commonly used disinfectants. In Venezuela there have recently been many non-tuberculous mycobacteria soft tissue infections after minor surgical procedures, some apparently related to the use of a commercial disinfectant based on a Quaternary Ammonium Compound. We studied the activity of this and other quaternary ammonium compounds on different non-tuberculous mycobacteria by transforming the mycobacteria with a dnaA-gfp fusion and then monitoring fluorescence to gauge the capacity of different quaternary ammonium compounds to inhibit bacterial growth. The minimum inhibitory concentration varied for the different quaternary ammonium compounds, but M. chelonae and M. abscessus were consistently more resistant than M. smegmatis, and M. terrae more resistant than M. bovis BCG.