Real-time PCR for differential quantification of CVI988 vaccine virus and virulent strains of Marek’s disease virus
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- 作者：Susan J. Baigenta ; sue.baigent@outlook.com" class="auth_mail" title="E-mail the corresponding author ; Venugopal K. Naira ; venugopal.nair@pirbright.ac.uk" class="auth_mail" title="E-mail the corresponding author ; Hervé ; Le Galludecb ; Herve.Legalludec@zoetis.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：40-Ct ; cycle threshold value in real-time PCR subtracted from 40 ; BAC ; bacterial-artificial-chromosome ; dpc ; days post challenge ; dpv ; days post vaccination ; FAM-BHQ1 ; probe with carboxyfluorescein (FAM) reporter fluorochrome and Black Hole Quencher ; LOD ; limit of detection by q-PCR ; ovo ; chicken ovotransferrin gene ; PBL ; peripheral blood lymphocytes ; pp38-CVI ; PCR probe specific for CVI988 pp38 gene sequence ; pp38-Vir ; PCR probe specific for virulent MDV pp38 gene sequence ; q-PCR ; real-time qua
- 刊名：Journal of Virological Methods
- 出版年：2016
- 出版时间：July 2016
- 年：2016
- 卷：233
- 期：Complete
- 页码：23-36
- 全文大小：1390 K
文摘
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Developed, optimised and validated q-PCR to distinguish CVI988 and virulent MDV.
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Based on differential detection of single nucleotide polymorphism in pp38 gene.
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Specific, sensitive, efficient, reproducible assay with no false-positive results.
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Accurate differential quantification over biological range of virus levels.
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Replication of CVI988 and virulent MDV examined in chicken feathers and blood.