A new real-time RT-qPCR assay for the detection, subtyping and quantification of human respiratory syncytial viruses positive- and negative-sense RNAs
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- 作者：Manel Essaidi-Laziosia ; b ; Manel.Essaidi-Laziosi@hcuge.ch" class="auth_mail" title="E-mail the corresponding author ; Matthieu Lyonb ; Matthieu.Lyon@etu.unige.ch" class="auth_mail" title="E-mail the corresponding author ; Aline Maminb ; Aline.Mamin@hcuge.ch" class="auth_mail" title="E-mail the corresponding author ; Mé ; lanie Fernandes Rochab ; Melanie.fernandesRocha@hcuge.ch" class="auth_mail" title="E-mail the corresponding author ; Laurent Kaiserb ; Laurent.Kaiser@hcuge.ch" class="auth_mail" title="E-mail the corresponding author ; Caroline Tapparela ; b ; Caroline.Tapparel@hcuge.ch" class="auth_mail" title="E-mail the corresponding author
- 关键词：6-FAM ; 6-carboxyfluorescein ; BHQ ; black hole quencher ; CT ; threshold cycle ; hMPV ; human metapneumovirus ; hPIV ; human parainfluenza virus ; L-LOD ; lower limit of detection ; LNA ; locked nucleic acid ; MGB-NFQ ; minor-groove binder non-fluorescence quencher ; QCMD ; Quality Control Molecular Diagnostics ; RSV ; human respiratory syncytial virus ; RT-qPCR ; real-time quantitative PCR ; U- and L- LOQ ; upper and lower limit of quantification
- 刊名：Journal of Virological Methods
- 出版年：2016
- 出版时间：September 2016
- 年：2016
- 卷：235
- 期：Complete
- 页码：9-14
- 全文大小：356 K
文摘
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New quantitative RT-qPCR assays for RSV-A and -B were developed.
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Both assays allow rapid detection, quantification and subtyping of RSV.
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The efficiency test was validated with in vitro transcripts and clinical samples.
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Comparable sensitivity was observed with RSV positive and negative sense RNAs.
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The assays provide a useful tool for both RSV diagnostics and research.