Single nucleotide polymorphism discrimination with and without an ethidium bromide intercalator

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• 作者：Renzo A. Fenati^a ; Ashley R. Connolly^a ; Amanda V. Ellis^a ; ^b ; ^{amanda.ellis@flinders.edu.au} ; ^{amanda.ellis@unimelb.edu.au}
• 关键词：Nucleotide polymorphism ; Strand displacement ; Fluorescence quenching ; Genotyping ; Kinetics
• 刊名：Analytica Chimica Acta
• 出版年：2017
• 出版时间：15 February 2017
• 年：2017
• 卷：954
• 期：Complete
• 页码：121-128
• 全文大小：933 K
• 卷排序：954

文摘

Fluorophores and DNA intercalators effect the rate of toehold-mediated strand displacement. Ethidium bromide had a destabilizing effect on mismatches that contained cytosine. A cationic fluorophore and Black Hole Quencher 1 strand displacement system was 2–3 times faster than a FRET system. This enabled SNP detection using toehold-mediated strand displacement in 15 min.