In vivo effects of LCO soluble fraction on immune-related functions and gene transcription in the Pacific oyster, Crassostrea gigas (Thunberg)

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• 作者：Anne Bado-Nilles ; Tristan Renault ; Nicole Faury ; Sté ; phane Le Floch ; Claire Quentel ; Michel Auffret ; Hé ; lè ; ne Thomas-Guyon
• 关键词：Crassostrea gigas ; Light cycle oil ; Immune parameters ; Bioaccumulation ; Gene transcription
• 刊名：Aquatic Toxicology
• 出版年：2010
• 出版时间：5 May 2010
• 年：2010
• 卷：97
• 期：3
• 页码：196-203
• 全文大小：392 K

文摘

The effects of a soluble fraction of light cycle oil (LCO) on haemocyte parameters, phenoloxidase (PO) activity and mRNA expression of immune-related genes, in the Pacific oyster, Crassostrea gigas, were tested after seven days of exposure and two weeks of recovery period. Five polycyclic aromatic hydrocarbons (PAHs) out of ten detected in tank water had bioaccumulated at the end of the contamination period. The concentration of PAHs in oyster tissues decreased during the recovery period and 14 days after the exposure, 69 % of bioaccumulated PAHs were detected in contaminated oysters. The exposure induced severe oyster mortality (21 % ), external and internal green colouration of the shell and a significant decrease of PO activity. The mRNA expression of several genes was altered. As a conclusion, a modulation of immune-related parameters was demonstrated using three different approaches, namely cellular (flow cytometry), biochemical (spectrophotometry) and genomics (gene transcription) in oysters after contact with soluble fraction of LCO.