Sarniensine, a mesembrine-type alkaloid isolated from Nerine sarniensis, an indigenous South African Amaryllidaceae, with larvicidal and adulticidal activities against Aedes aegypti

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• 作者：Marco Masi^a ; Alet E. van der Westhuyzen^b ; Nurhayat Tabanca^c ; ^d ; Marco Evidente^a ; Alessio Cimmino^a ; Ivan R. Green^b ; Ulrich R. Bernier^d ; James J. Becnel^d ; Jeffrey R. Bloomquist^c ; Willem A.L. van Otterlo^b ; Antonio Evidente^a ; ^{evidente@unina.it}
• 关键词：Nerine sarniensis ; Amaryllidaceae alkaloids ; Sarniensine ; Larvicide and adulticide activities ; Natural pesticide ; Aedes aegypti
• 刊名：Fitoterapia
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：116
• 期：Complete
• 页码：34-38
• 全文大小：371 K
• 卷排序：116

文摘

A new mesembrine-type alkaloid, named sarniensine, was isolated together with tazettine, lycorine, the main alkaloid, and 3-epimacronine from Nerine sarniensis, with the last two produced for the first time by this plant. This Amaryllidaceae, which is indigenous of South Africa, was investigated for its alkaloid content, because the organic extract of its bulbs showed strong larvicidal activity with an LC50 value of 0.008 μg μL− 1 against first instar Aedes aegypti larvae and with an LD50 value 4.6 μg/mosquito against adult female Ae. aegypti, which is the major vector for dengue, yellow fever and the Zika virus. The extract did not show repellency at MED value of 0.375 mg cm2 against adult Ae. aegypti. Sarniensine was characterized using spectroscopic and chiroptical methods as (3aR,4Z,6S,7aS)-6-methoxy-3a-(2′-methoxymethyl-benzo [1,3]dioxol-1'-yl)-1-methyl-2,3,3a,6,7,7a-hexahydro-1H-indole. It was less effective against larva at the lowest concentration of 0.1 μg μL− 1, however it showed strong adulticidal activity with an LD50 value of 1.38 ± 0.056 μg mosquito− 1.