Infection and transmission dynamics of rKSHV.219 in primary endothelial cells

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• 作者：Hannah C. Jeffery ; Rachel L. Wheat ; David J. Blackbourn ; Gerard B. Nash ; Lynn M. Butler
• 关键词：Endothelial cell ; Kaposi's sarcoma-associated herpes virus ; Rkshv.219
• 刊名：Journal of Virological Methods
• 出版年：2013
• 出版时间：October, 2013
• 年：2013
• 卷：193
• 期：1
• 页码：251-259
• 全文大小：1987 K

文摘

Kaposi's sarcoma-associated herpesvirus (KSHV) is the aetiologic agent of Kaposi's sarcoma (KS), a tumour of endothelial cell origin. The study of KS development was aided by the generation of a recombinant GFP (latent)/RFP (lytic)-expressing KSHV (rKSHV.219) by . In this study the first data characterising primary endothelial cell infection and transmission with this virus is presented. Infection was predominantly latent and the percentage of GFP-positive cells increased over time. Neither horizontal transmission of infection, nor cellular proliferation, explained this increase. Analysis of latency-associated nuclear antigen (LANA-1) expression revealed that a threshold level of infection was required for GFP expression early post infection. At later time points GFP correlated more closely with LANA-1 expression, likely due to the accumulation of GFP over time. This study provides methodological guidance for the use of rKSHV.21. In addition, it highlights potential problems associated with the use of fluorescent proteins as markers of viral infection.