Electrochemical Effects after Transarterial Chemoembolization in Combination with Percutaneous Irreversible Electroporation: Observations in an Acute Porcine Liver Model

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• 作者：Dominik Vollherbst ; MD<sup>asup><sup> ; sup><sup>dsup> ; Robert C. Bertheau ; MD<sup>asup> ; Stefan Fritz ; MD<sup>bsup> ; Carolin Mogler ; MD<sup>csup> ; Hans-Ulrich Kauczor ; MD<sup>asup> ; Eduard Ryschich ; MD<sup>bsup> ; Boris A. Radeleff ; MD<sup>asup> ; Philippe L. Pereira ; MD<sup>dsup> ; Christof M. Sommer ; MD<sup>asup><sup> ; sup><sup>esup><sup> ; sup><sup>christof.sommer@med.uni-heidelberg.de" class="auth_mail" title="E-mail the corresponding authorsup>
• 关键词：H/E ; hematoxylin/eosin ; HPLC ; high-performance liquid chromatography ; IRE ; irreversible electroporation ; TUNEL ; terminal deoxynucleotidyl transferase&ndash ; mediated deoxyuridine triphosphate biotin nick-end labeling ; MG ; Masson&ndash ; Goldner ; RE ; reversible electroporation
• 刊名：Journal of Vascular and Interventional Radiology
• 出版年：2016
• 出版时间：June 2016
• 年：2016
• 卷：27
• 期：6
• 页码：913-921.e2
• 全文大小：6117 K

文摘

To evaluate the effects of combined use of transarterial chemoembolization and irreversible electroporation (IRE) for focal tissue ablation in an acute porcine liver model.

sSec_2">Materials and Methods

sp0090">Two established interventional techniques were combined: IRE with zones of irreversible and reversible electroporation and chemoembolization with microspheres, iodized oil, and doxorubicin. IRE was performed before chemoembolization in two pigs (pigs 1 and 2; IRE/chemoembolization group), chemoembolization was performed before IRE in two pigs (pigs 3 and 4; chemoembolization/IRE group), and only IRE was performed in two pigs (pigs 5 and 6). Five study groups were defined: IRE/chemoembolization (pigs 1 and 2), chemoembolization/IRE (pigs 3 and 4), IRE only (pigs 5 and 6), chemoembolization only (tissue outside the IRE zones in pigs 1&ndash;4), and control (untreated liver tissue outside the IRE zones in pigs 5 and 6). Animals were euthanized 2 hours after intervention. Size and shape of IRE zones on contrast-enhanced computed tomography, cell death on light microscopy, and doxorubicin tissue concentrations on chromatography and fluorescence microscopy were analyzed.

sSec_3">Results

sp0095">Size and shape of IRE zones were not significantly different (eg, P = .067 for volume). A histologic marker for irreversible cell death was positive in IRE/chemoembolization, chemoembolization/IRE, and IRE groups only in the macroscopically visible IRE zones. Doxorubicin tissue concentrations were not significantly different (P = .873). However, in the reversible electroporation (RE) zones, broad areas with intense intranuclear doxorubicin accumulation were observed in IRE/chemoembolization but not in chemoembolization/IRE and chemoembolization groups.

sSec_4">Conclusions

sp0100">IRE before chemoembolization enhances the intranuclear accumulation of doxorubicin in the RE zone.