Transcriptome response of wheat Norin 10 to long-term elevated CO<sub>2sub> under high yield field condition
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- 作者：Yue-bing LIN<sup>asup><sup> ; sup><sup>*sup><sup> ; sup><sup>paradelynn@163.com" class="auth_mail" title="E-mail the corresponding authorsup> ; Cheng-guo SHEN<sup>bsup><sup> ; sup><sup>*sup><sup> ; sup><sup>success.100081@gmail.com" class="auth_mail" title="E-mail the corresponding authorsup> ; Er-da LIN<sup>asup><sup> ; sup><sup>linerda@caas.cn" class="auth_mail" title="E-mail the corresponding authorsup> ; Xing-yu HAO<sup>csup> ; Xue HAN<sup>asup>
- 关键词：Norin 10 ; transcriptome response ; wheat ; long term elevated carbon dioxide ; GO enrichment
- 刊名：Journal of Integrative Agriculture
- 出版年：2016
- 出版时间：September 2016
- 年：2016
- 卷：15
- 期：9
- 页码：2142-2152
- 全文大小：1285 K
文摘
The increasing atmospheric carbon dioxide concentration, caused by fossil fuel combustion and deforestation, plays an important role in plant growth and development. Wheat, as a major staple crop, adapts to climate change by tuning its inherent molecular mechanism, which is not well understood. The present study employed the RNA-Seq method to generate transcriptome profiles of the wheat Norin 10 in response to elevated CO<sub>2sub> in comparison with ambient CO<sub>2sub>. The 10 895 787 high-quality clean reads of Norin 10 were assembled de novo using Trinity (without a reference genome) resulting in a total of 18 206 candidate transcripts with significant BLAST matches. GO enrichment analysis of Norin 10 at different CO<sub>2sub> concentrations showed that some functional genes related to plastids, precursor metabolites, and energy, thylakoid and photosynthesis were apparently enriched at elevated CO<sub>2sub> (550 µmol mol<sup>−1sup>) in contrast to that at ambient CO<sub>2sub> (400 µmol mol<sup>−1sup>); these findings were further confirmed by RT-PCR analysis. The findings demonstrated the specific effects of elevated CO<sub>2sub> during long-term period in free air CO<sub>2sub> enrichment (FACE) on transcriptome response of the high yielding wheat variety, Norin 10, which has a large spike.