Inhibition of Raf/MAPK Signaling inXenopusOocyte Extracts by Raf-1-Specific Peptides

详细信息    查看全文

• 作者：Radziwill ; Gerald ; Steinhusen ; Ulrike ; Aitken ; Alastair ; Moelling ; Karin
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：1996
• 出版时间：October 3, 1996
• 年：1996
• 卷：227
• 期：1
• 页码：20-26
• 全文大小：199 K

文摘

Raf-1 is an upstream element of the mitogen-activated protein kinase (MAPK) pathway which leads to cell proliferation and differentiation. In this study Raf-1 derived peptides comprising the conserved amino acid residues Arg89 and Ser259, involved in binding of activated Ras and 14-3-3 proteins, respectively, were shown to interfere with MAPK activation in extracts from immatureXenopusoocytes. Lipids prepared from oocyte extracts can stimulate MAPK in a Ras- and protein kinase C-independent manner. This lipid-induced MAPK activation is blocked by a Raf-1 derived peptide comprising Ser259.