A self-assembling peptide (SAP) was covalently immobilized of on a flat silicon surface.
A physisorbed SAP layer was grown on top of the covalently immobilized peptide layer.
Molecular order and orientation of the peptide overlayer on the flat silicon surface were determined by NEXAFS spectroscopy.
RAIR spectroscopy allowed to determine that the peptide secondary β-sheet structure is retained.