Digestion pattern of chia seed oil (CSO) in its protein (CPI) and gum (CSG) coacervate was studied. Hydrolysis of CPI in gastric and intestinal stages produced cationic and anionic peptides. Most of encapsulated CSO (38–84%) was released in gastric stage. CPI-CSG shell was better able to deliver intact CSO to intestinal stage for digestion. Digestion of CSO occurred only in the intestinal stage due to the action of lipase.