In vitro and in vivo effects of neem tree (Azadirachta indica A. Juss) products on larvae of the sheep nose bot fly (Oestrus ovis L. D铆ptera: Oestridae)

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• 作者：R. Cepeda-Palacios ; R. Serv铆n ; J.M. Ram铆rez-Ordu帽a ; F. Ascencio ; P. Dorchies ; C.E. Angulo-Valadez
• 关键词：Neem (Azadirachta indica A. Juss) ; O. ovis ; Larval development ; Larval mortality ; Sheep
• 刊名：Veterinary Parasitology
• 出版年：24 February, 2014
• 年：2014
• 卷：200
• 期：1-2
• 页码：225-228
• 全文大小：363 K

文摘

Two studies were carried out in order to test the effects of neem tree extracts (Azadirachta indica A. Juss) on sheep bot fly larvae (Oestrus ovis L. Diptera: Oestridae). First, aqueous extracts from neem seeds (ASNE) at 0, 5 y 10% (w/v) concentrations were tested on larval mortality in vitro. In a second study, the effect of oral administration with neem seed meal (0, 100 y 200 mg/kg) and neem leaves (1% of diet) on number of larvae found at necropsy and larval development was evaluated in experimentally O. ovis-infected sheep. Results in Experiment 1 showed a significant (P < 0.05) effect of ASNE on time to L1 mortality in a dosis-dependent manner. In Experiment 2, oral administration of seeds or leaves did not affect the number of larvae found at necropsy of the sheep, but interfered with larval development and there was a tendency to reduce larval weight at the end of the infection period (55 d).