Development and physico-chemical characterization of cyclodextrin–DNA complexes loaded liposomes

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• 作者：Guilherme D. Tavares ; Cristiane M. Viana ; José ; G.V.C. Araú ; jo ; Gilson A. Ramaldes ; Wâ ; nia S. Carvalho ; Jorge L. Pesquero ; José ; M.C. Vilela ; Margareth S. Andrade and Mô ; nica C. de
• 刊名：Chemical Physics Letters
• 出版年：2006
• 出版时间：5 October 2006
• 年：2006
• 卷：429
• 期：4-6
• 页码：507-512
• 全文大小：287 K

文摘

In the present study, anionic and pH-sensitive liposomes containing DNA were developed and characterized. These liposomes were obtained by binding the DNA with 6-monodeoxy-6-monoamine-β-cyclodextrin (Am-β-CD). This complex was encapsulated into the liposomes, which were characterized by encapsulation rate, diameter, zeta potential, and atomic force microscopy. The binding between Am-β-CD and the DNA was higher as of the +/− charge ratio. The amount of DNA encapsulated was approximately 10–14 μg/mL. The mean diameter and zeta potential were 186.0 nm and −56 mV, respectively. Liposomes which did not contain the complex were more prone to collapse over the mica surface. The vesicles containing the complex presented a narrower size distribution.