Cloning and expression of phosphoenolpyruvate carboxykinase from a cestode parasite and its solubilization from inclusion bodies using l-arginine
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- 作者：Asim K. Dutta ; Ramnath ; Barilin Dkhar ; Veena Tandon ; Bidyadhar Das
- 关键词：PEPCK ; Raillietina echinobothrida ; Inclusion bodies ; l-Arginine
- 刊名：Protein Expression and Purification
- 出版年：2016
- 出版时间：September 2016
- 年：2016
- 卷：125
- 期：Complete
- 页码：61-67
- 全文大小：915 K
文摘
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- <p id="p0005">The ORF of PEPCK from a parasite was cloned into pGEX-4T3, which expressed as IBs.p>
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- <p id="p0010">Addition of 2 M
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- <p id="p0015">A yield of ∼73 mg of purified rePEPCK was obtained per 1 L of culture.p>
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- <p id="p0020">The specific activity and Kmapp were 21 U/mg of protein and 43 μM, respectively.p>