β-xylosidase from Selenomonas ruminatium was expressed in Pichia pastoris using alcohol oxidase I promoter.
The synthetic gene was optimized based on Pichia pastoris codon preference.
This is the first report on the cloning and expression of a β-xylosidase gene from GH family 43 in Pichia pastoris.
Expression of β-xylosidase was increased three times by optimization of growth and induction conditions in shake flask.
The recombinant xylosidase showed higher turnover rate rather than most xylosidase reported previously.