A step-by-step protocol for assaying protein carbonylation in biological samples
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- 作者：Graziano Colomboa ; graziano.colombo@unimi.it" class="auth_mail" title="E-mail the corresponding author ; Marco Clericia ; Maria Elisa Garavagliaa ; Daniela Giustarinib ; Ranieri Rossib ; Aldo Milzania ; Isabella Dalle-Donnea
- 关键词：2D-GE ; two-dimensional gel electrophoresis ; 4-HNE ; 4-hydroxynonenal ; ARP ; aldehyde-reactive probe ; BHZ ; biotin-hydrazide ; CHAPS ; 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate ; DMF ; dimethyl formammide ; DMSO ; dimethyl sulfoxide ; DNPH ; 2 ; 4-dinitrophenylhydrazine ; DTT ; dithiotreitol ; ECL ; enhanced chemiluminescence ; ELISA ; enzyme-linked immunosorbent assay ; FITC ; fluorescein isothiocyanate ; FTC ; fluorescein-5-thiosemicarbazide ; HGFs ; human gingival fibroblasts ; HPLC ; high performance li
- 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
- 出版年：2016
- 出版时间：15 April 2016
- 年：2016
- 卷：1019
- 期：Complete
- 页码：178-190
- 全文大小：1470 K
文摘
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Protein carbonylation is a frequent irreversible oxidative protein modification.
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Many cheap and user-friendly protocols are available for evaluating carbonylation.
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We describe and comment various step-by-step protocols that use labelling molecules.