Signaling States of a Short Blue-Light Photoreceptor Protein PpSB1-LOV Revealed from Crystal Structures and Solution NMR Spectroscopy
详细信息 查看全文
- 作者：Katrin Rö ; llen1 ; &dagger ; ; Joachim Granzin1 ; &dagger ; ; Vineet Panwalkar1 ; &dagger ; ; Vladimir Arinkin1 ; Raj Rani2 ; 5 ; Rudolf Hartmann1 ; Ulrich Krauss2 ; Karl-Erich Jaeger2 ; 3 ; Dieter Willbold1 ; 4 ; Renu Batra-Safferling1 ; r.batra-safferling@fz-juelich.de" class="auth_mail" title="E-mail the corresponding author
- 关键词：LOV ; light&ndash ; oxygen&ndash ; voltage ; PAS ; Per-ARNT-Sim ; FMN ; flavin mononucleotide ; 2D ; two-dimensional ; HSQC ; heteronuclear single-quantum coherence ; NOE ; nuclear Overhauser enhancement ; τm ; rotational correlation time ; DSS ; 2 ; 2-dimethyl-2-silapentane-5-sulfonate
- 刊名：Journal of Molecular Biology
- 出版年：2016
- 出版时间：25 September 2016
- 年：2016
- 卷：428
- 期：19
- 页码：3721-3736
- 全文大小：1689 K
文摘
- •
Comparison of crystal structures of PpSB1-LOV in dark and light states
- •
Dimer interface and the C-terminal Jα-helix show major structural rearrangements.
- •
A ~ 29° rotation between the two protein chains gated by light
- •
Extensive NMR solution studies reveal light-induced conformational changes.
- •
We propose a rotary switch mechanism for the activation.