mTOR/p70S6K activation in area CA3 are involved in long term memory formation. Rapamycin inhibited mTOR/p70S6K activation at 4 h and impaired long term memory at 24 h. Scopolamine activated mTOR/p70S6K at 1 h and impaired short but not long term memory. Mecamylamine reduced the scopolamine-induced increase of mTOR/p70S6K activation at 1 h. Rapamycin increased mTORC2 activation in microglial cells in Stratum Radiatum.