The effects of iloprost on colonic anastomotic healing in rats under obstructive ileus conditions

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• 作者：Georgios Galanopoulos ; MD ; PhD^a ; ¹ ; Dimitrios Raptis ; MD ; PhD^a ; ^b ; ¹ ; ^{draptis@hotmail.com" class="auth_mail} ; Manousos-Georgios Pramateftakis ; MD ; PhD^a ; Ioannis Mantzoros ; MD ; PhD^a ; Ioannis Kanellos ; MD ; PhD^a ; Charalambos Lazarides ; MD ; PhD^a
• 关键词：Iloprost ; Ileus ; Obstruction ; Colonic anastomosis ; Colon cancer
• 刊名：Journal of Surgical Research
• 出版年：1 June, 2014
• 年：2014
• 卷：189
• 期：1
• 页码：22-31
• 全文大小：710 K

文摘

Background

The aim of this study was to investigate the effects of iloprost, on colonic anastomotic healing in rats, under obstructive ileus conditions.

Materials and methods

Eighty male Albino rats were randomized into four groups of 20 animals each. They underwent colonic resection followed by an inverted anastomosis. The rats of group 1 (control) and group 2 (ileus) received 3聽mL of saline 0.9% intraperitoneally and those of group 3 (iloprost), and group 4 (ileus聽+聽iloprost) iloprost (2聽渭g/kg of body weight), immediately postoperatively and daily until the day of sacrifice. Each group was further divided into two equal subgroups, depending on the day of sacrifice. The animals of subgroup 鈥渁鈥?were sacrificed on the fourth postoperative day, whereas those of 鈥渂鈥?on the eighth day. Macroscopic and histologic assessment was performed, whereas anastomotic bursting pressures and the tissue concentrations in hydroxyproline and collagenase I were evaluated.

Results

Means of bursting pressure, neoangiogenesis, fibroblast activity, and hydroxyproline concentration were significantly increased in group 4 compared with group 2. In addition, on the fourth postoperative day, the inflammatory cell infiltration and the collagenase I concentration were significantly decreased in group 4 compared with group 2. Moreover, on the eighth postoperative day, collagen deposition was significantly increased in group 4 compared with group聽2.

Conclusions

Iloprost after intraperitoneal administration reverses the negative effect of obstructive ileus. It promotes not only the angiogenic activity but also collagen formation, resulting in increased bursting pressures on the fourth and eighth postoperative days.