- 作者：Hye Young Kim ; khyey@kfri.re.kr ; Kyong Kim
- 关键词：Korean red ginseng (KRG) ; Goto-Kakizaki rats ; Diabetes ; Insulin action ; Insulin secretion ; ¦Â-Cell mass
- 刊名：Journal of Ethnopharmacology
- 出版年：2012
- 出版时间：26 June, 2012
- 年：2012
- 卷：142
- 期：1
- 页码：53-58
- 全文大小：738 K
Ethnopharmacological relevance
Korean red ginseng (KRG) has long history as herbal remedy for antidiabetic effect.Aim of the study
To study molecular mechanisms by which KRG ameliorates diabetes mellitus, we investigated whether the supplementation with the aqueous extract of KRG as a dietary admixture (1 % , w/w) regulates the expressions of signaling molecules that are associated with insulin action, insulin secretion and pancreatic ¦Â-cell mass in spontaneously diabetic Goto-Kakizaki (GK) rats.
Methods
An aqueous extract of KRG was supplemented for the estimated dosage to be 0.2 g/kg rat/day beginning at 5 weeks of age for 12 weeks in male GK rats. Plasma glucose levels were measured every 4 weeks. The expressions of signaling molecules that are associated with insulin action, insulin secretion and ¦Â-cell mass in tissues were determined by Western blotting.
Results
The 12-week supplementation with KRG significantly (P<0.05) decreased blood glucose compared to control. It up-regulated the expression of glucose transporter (GLUT) 4 in adipose tissue, and down-regulated the expression of protein tyrosine phosphatases (PTP)-1B in adipose tissue and skeletal muscle. It also up-regulated the expression of insulin and down-regulated the expression of uncoupling protein (UCP) 2, Bax and poly (ADP-ribose) polymerase (PARP) in pancreas.
Conclusions
These results suggest that GLUT4, PTP-1B, insulin, UCP2, Bax and PARP may be the primary targets of KRG that result in increase in insulin action and in insulin secretion, and decrease in ¦Â-cell mass, and that cause the normalization in glucose homeostasis.