- 作者：Seema Taprial ; Deepak Kashyap ; Vineet Mehta ; Sunil Kumar ; Dinesh Kumar ; dineshbarbola@yahoo.co.in
- 关键词：Anti-fertility ; Anti-implantation ; Estrogenic ; Hydroalchoholic extract ; Michelia champaca
- 刊名：Journal of Ethnopharmacology
- 出版年：2013
- 出版时间：3 June, 2013
- 年：2013
- 卷：147
- 期：3
- 页码：671-675
- 全文大小：252 K
Ethnopharmacological relevance
Michelia champaca L. (family: Magnoliaceae), commonly known as Champa [Hindi], is traditionally used for fertility regulation by the women of Chhattisgarh state in India. No scientific evidence regarding the antifertility effect of this plant is available till date.Aim of the study
To study the anti-fertility effect of hydroalcoholic leaf extract of Michelia champaca Linn. in female rats.
Materials and methods
The antifertility activity of the extract (HAEMC) administered at dose levels (100 and 200 mg/kg body weight, p.o.) was evaluated in two experimental animal models i.e. antiimplantation activity in female wistar rats and esterogenic/antiestrogenic activity in ovariectomized female rats. In anti-implantation activity, the extract (200 and 400 mg/kg body weight, p.o.) was administered to female rats from 1 to 7 days of pregnancy and on 10th day, laprotomy was performed to count the no. of implants. For estrogenic/anti-estrogenic activity, ovariectomized female rats were administered with the extract at both the doses alone as well as along with 17¦Á-ethinyl estradiol (1 ¦Ì/rat/day) for 7 consecutive days. On the 8th day, all animals were sacrificed and blood serum was further processed for the estimation of biochemical parameters such as estrogen level, alkaline phosphates, cholesterol, tryglycerides, total protein etc.
Results
The extract (HAEMC) showed significant (p<0.01) 49.95 % and 71.03 % antiimplantation activities at 100 and 200 mg/kg doses respectively. The extract also exhibited significant (p<0.01) estrogenic activity as evidenced by increase in body weight, uterine weight, increased thickness and height of endometrium, vaginal cornification and significant (p<0.01) increase in estrogen, cholesterol, alkaline phosphate and triglycerides levels at higher dose when administered alone as well as along with ethinyl estradiol. Phytochemical screening showed the presence of steroids, flavonoids and alkaloids in the extract.
Conclusions
Hydroalchoholic extract of Michelia champaca leaves possesses significant antifertility effect which might be due to the inhibition of implantation and estrogenic effect which in turn might be due to the presence of some phytoconstituents in the plant.