Avian haemosporidian parasites (Haemosporida): A comparative analysis of different polymerase chain reaction assays in detection of mixed infections
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- 作者：Rasa Bernotienė ; rasab@ekoi.lt" class="auth_mail" title="E-mail the corresponding author ; Vaidas Palinauskas vaidas@ekoi.lt" class="auth_mail" title="E-mail the corresponding author ; Tatjana Iezhova tatjana@ekoi.lt" class="auth_mail" title="E-mail the corresponding author ; Dovilė Murauskaitė d.murauskaitei@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Gediminas Valkiūnas gedvalk@ekoi.lt" class="auth_mail" title="E-mail the corresponding author
- 关键词：Avian malaria ; Polymerase chain reaction ; Mixed infection ; Detectability ; Haemoproteus ; Plasmodium
- 刊名：Experimental Parasitology
- 出版年：2016
- 出版时间：April 2016
- 年：2016
- 卷：163
- 期：Complete
- 页码：31-37
- 全文大小：369 K
文摘
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Sensitivity of 5 different PCR assays in detection of mixed haemosporidian infections was studied.
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Each PCR assay remarkably underestimate haemosporidian diversity.
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Application of 3–5 different PCR assays in parallel detect the majority of mixed infections.
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Preferences of different PCR assays were determined in haemosporidian diagnostics.