Laccase/HBT and laccase-CBM/HBT treatment of softwood kraft pulp: Impact on pulp bleachability and physical properties

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• 作者：Holy Ravalason ; Fr¨¦d¨¦rique Bertaud ; Isabelle Herpo?l-Gimbert ; Val¨¦rie Meyer ; Katia Ruel ; Jean-Paul Joseleau ; Sacha Grisel ; Caroline Oliv¨¦ ; Jean-Claude Sigoillot ; Michel Petit-Conil
• 关键词：Laccase ; CBM ; Biobleaching ; Softwood kraft pulp ; Chlorine dioxide
• 刊名：Bioresource Technology
• 出版年：2012
• 出版时间：October, 2012
• 年：2012
• 卷：121
• 期：Complete
• 页码：68-75
• 全文大小：471 K

文摘

Pycnoporus cinnabarinus laccase and a chimeric laccase-CBM were applied in softwood kraft pulp biobleaching in the presence of 1-hydroxybenzotriazole (HBT). The presence of CBM could enhance the laccase biobleaching potential as a decrease in the enzymatic charge and chlorine dioxide consumption, as well as an increase in pulp brightness were observed. Laccase/HBT treatment could be improved by increasing oxygen pressure from 1 to 3 bar and pulp consistency from 5 % to 10 % . Conversely, under the same conditions, no improvement of laccase-CBM/HBT treatment was observed, indicating a different behavior of both systems. However, laccase-CBM/HBT treatment led to a better preservation of pulp properties. This effect was probably due to fiber surface modifications involving the action of the CBM. Transmission electron microscopy examination of pulp fibers indicated a retention of laccase-CBM inside the pulp fibers due to CBM binding and an increased external microfibrillation of the fibers due to enzymatic treatments.