Intra- and inter-laboratory reproducibility and accuracy of the LuSens assay: A reporter gene-cell line to detect keratinocyte activation by skin sensitizers
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- 作者：Tzutzuy Ramireza ; Nadine Steina ; Alexandra Aumanna ; Tina Remusb ; Amber Edwardsc ; Kimberly G. Normand ; Cindy Ryane ; Jackie E. Baderd ; Markus Fehrb ; Florence Burlesonc ; Leslie Foertsche ; Xiaohong Wange ; Frank Gerbericke ; Paul Beilsteinb ; Sebastian Hoffmannf ; Annette Mehlingg ; Bennard van Ravenzwaaya ; Robert Landsiedela ; robert.landsiedel@basf.com" class="auth_mail" title="E-mail the corresponding author
- 刊名：Toxicology in Vitro
- 出版年：2016
- 出版时间：April 2016
- 年：2016
- 卷：32
- 期：Complete
- 页码：278-286
- 全文大小：244 K
文摘
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Keratinocyte activation is a key event of the skin sensitization AOP.
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ARE–Nrf2-luciferase methods are accepted under OECD 442D to address this key event.
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LuSens is a human keratinocyte line with an ARE–Nrf2-luciferase reporter.
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Results of an inter-laboratory evaluation conducted in five laboratories
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Reproducibility of 100% and accuracy in identifying skin sensitizers over 80%