Application of octadecanethiol self-assembled monolayer to cholesterol biosensor based on surface plasmon resonance technique
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- 作者：Sunil K. Arya ; Pratima R. Solanki ; Ravindra P. Singh ; Manoj K. Pandey ; Monika Datta and Bansi D. Malhotra
- 关键词：Cholesterol ; Cholesterol oxidase (ChOx) ; Surface plasmon resonance (SPR) ; Octadecanethiol (ODT) ; 1-Fluoro-2-nitro-4-azidobenzene (FNAB)
- 刊名：Talanta
- 出版年：2006
- 出版时间：15 June 2006
- 年：2006
- 卷：69
- 期：4
- 页码：918-926
- 全文大小：385 K
文摘
Octadecanethiol (ODT) self-assembled monolayer (SAM) prepared onto gold-coated glass plate has been modified by using nitrene reaction of 1-fluoro-2-nitro-4-azidobenzene (FNAB) that further covalently binds to cholesterol oxidase (ChOx) via thermal reaction. FNAB acts as a bridge (cross-linker) between SAM and ChOx. The ChOx/FNAB/ODT/Au electrode thus fabricated has been characterized using contact angle (CA) measurements, UV–vis spectroscopy, electrochemical techniques and X-ray photoelectron spectroscopy (XPS) technique, respectively. This ChOx/FNAB/ODT/Au bioelectrode has been utilized for estimation of cholesterol in solution using surface plasmon resonance (SPR) technique. This SPR based cholesterol biosensor has linearity from 50 to 500 mg/dl of cholesterol in solution with lower detection limit of 50 mg/dl and shelf life of about 2 months when stored at 4 °C.