In vitro model to estimate Edwardsiella tarda-macrophage interactions using RAW264.7 cells

详细信息    查看全文

• 关键词：Edwardsiella tarda ; Macrophage ; RAW264.7 ; Cell interaction
• 刊名：Fish & Shellfish Immunology
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：60
• 期：Complete
• 页码：177-184
• 全文大小：2106 K
• 卷排序：60

文摘

RAW264.7 cells may be considered as a cell model for study on interactions between E. tarda and macrophages. MOIs of 10:1 and 100:1 are recommended for using E. tarda-invaded RAW264.7 cells. E. tarda could induce the apoptosis in RAW264.7 cells through the involvement of caspase-3. Once inside macrophage, numberous E. tarda could be destroyed within the phagolysosomes. E. tarda who successfully subvert phagocyte defenses could replicate within the vacuolar-like compartment in macrophages.