Variation in copy number of the 28S rDNA of Aspergillus fumigatus measured by droplet digital PCR and analog quantitative real-time PCR

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• 作者：Alexandre Alanio^a ; ^b ; ^c ; Aude Sturny-Leclè ; re^c ; Marion Benabou^d ; Nicolas Guigue^a ; Sté ; phane Bretagne^a ; ^b ; ^c ; ^{stephane.bretagne@aphp.fr" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Droplet digital PCR ; Real-time quantitative PCR ; rDNA ; Aspergillus fumigatus ; Copy number variation
• 刊名：Journal of Microbiological Methods
• 出版年：2016
• 出版时间：August 2016
• 年：2016
• 卷：127
• 期：Complete
• 页码：160-163
• 全文大小：686 K

文摘

Droplet digital PCR (ddPCR) after DNA digestion yielded a 28S rDNA copy number of 61 to 86 copies/genome when testing 10 unrelated Aspergillus fumigatus isolates, higher than with quantitative PCR. Unfortunately, ddPCR after DNA digestion did not improve the sensitivity of our PCR assay when testing serum patients with invasive aspergillosis.