Perkinsus sp. infecting oyster Crassostrea rhizophorae (Guilding, 1828) on the coast of Bahia, Brazil

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• 作者：Rosana Pinho Brandã ; o^a ; Guisla Boehs^a ; ^{guislaboehs@hotmail.com} ; Rachel Costa Sabry^b ; Liliane Oliveira Ceuta^a ; Mariane dos Santos Aguiar Luz^a ; Fernando Ramos Queiroga^c ; Patrí ; cia Mirella da Silva^c
• 关键词：Perkinsus ; Crassostrea rhizophorae ; Health ; Histology ; RFTM ; PCR
• 刊名：Journal of Invertebrate Pathology
• 出版年：2013
• 出版时间：February, 2013
• 年：2013
• 卷：112
• 期：2
• 页码：138-141
• 全文大小：543 K

文摘

This study investigated the occurrence of the protozoan Perkinsus in the oyster Crassostrea rhizophorae on the coast of Bahia State, Brazil. The oysters (n = 900) were collected in February-March and July-August 2010. The Ray¡¯s fluid thioglycollate medium (RFTM) analysis of gills and rectum revealed hypnospores of Perkinsus sp. with a high mean prevalence (63 % ). The infection intensity varied from very light to advanced. The polymerase chain reaction confirmed Perkinsus in 87.2 % of the RFTM-positive oysters. Histological analysis showed trophozoites and schizonts phagocytized by hemocytes, mainly in the intestine and the stomach epithelium.