Development of a continuous l-lysine bioconversion system for cadaverine production

详细信息    查看全文

• 作者：Jung-Ho Kim^a ; ¹ ; Hyung-Min Seo^a ; ¹ ; Ganesan Sathiyanarayanan^a ; Shashi Kant Bhatia^a ; Hun-Suk Song^a ; Junyoung Kim^a ; Jong-Min Jeon^a ; Jeong-Jun Yoon^b ; Yun-Gon Kim^c ; Kyungmoon Park^d ; Yung-Hun Yang^a ; ^e ; ^{seokor@konkuk.ac.kr}
• 关键词：Cadaverine ; Bioconversion ; Immobilization ; Continuous production ; Packed-bed reactor
• 刊名：Journal of Industrial and Engineering Chemistry
• 出版年：2017
• 出版时间：25 February 2017
• 年：2017
• 卷：46
• 期：Complete
• 页码：44-48
• 全文大小：598 K
• 卷排序：46

文摘

Cadaverine, a five carbon diamine (1,5-diaminopentane), plays a role as a building block of polyamides and it can be made by fermentation or direct bioconversion. To improve its production by increasing reusability of immobilized enzyme and avoid separation of enzyme in bioconversion, a continuous l-lysine bioconversion process for cadaverine production has been developed. Various divalent cations, alginate concentrations, cell density with alginate and flow rate of feed were examined to maximize the lysine decarboxylase activity of the whole-cell immobilized beads. Under the selected conditions, 123 h of continuous cadaverine production has been performed and 5.5 L of 819 mM cadaverine were produced with 14 mL reactor resulting in 466.5 g of cadaverine. Cadaverine production was possible with small volume reactor maintaining relatively high concentration of substrate.