• 作者：L. Rivas ; A. Bl谩zquez ; F.J. Mu帽oz-Negrete ; S. L贸pez ; G. Rebolleda ; F. Dom铆nguez ; A. P茅rez-Esteban
• 关键词：Cultivo primario ; Diferenciació ; n celular ; Cé ; lulas epiteliales conjuntivales ; Membrana amnió ; tica humana ; Suero autó ; logo
• 刊名：Archivos de la Sociedad Espa帽ola de Oftalmolog铆a
• 出版年：January, 2014
• 年：2014
• 卷：89
• 期：1
• 页码：10-16
• 全文大小：1456 K

Objective

To evaluate primary cultures from human conjunctiva supplemented with fetal bovine serum, autologous serum, and platelet-rich autologous serum, over human amniotic membrane and lens anterior capsules.

Methods

One-hundred and forty-eight human conjunctiva explants were cultured in CnT50^庐 supplemented with 1, 2.5, 5 and 10% fetal bovine serum, autologous serum and platelet-rich autologous serum. Conjunctival samples were incubated at 37 掳C, 5% CO₂ and 95% HR, for 3 weeks.

Results

The typical phenotype corresponding to conjunctival epithelial cells was present in all primary cultures. Conjunctival cultures had MUC5AC-positive secretory cells, K19-positive conjunctival cells, and MUC4-positive non-secretory conjunctival cells, but were not corneal phenotype (cytokeratin K3-negative) and fibroblasts (CD90-negative).

Conclusions

Conjunctiva epithelial progenitor cells were preserved in all cultures; thus, a cell culture in CnT50^庐 supplemented with 1 to 5% autologous serum over human amniotic membrane can provide better information of epithelial cell differentiation for the conjunctival surface reconstruction.