Function of two novel single-CRD containing C-type lectins in innate immunity from Eriocheir sinensis

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• 作者：Ying Huang ; Xin Huang ; Zheng Wang ; Jing-Min Tan ; Kai-Min Hui ; Wen Wang ; ^{njnuwang@263.net" class="auth_mail} ; ^{wenwang@njnu.edu.cn" class="auth_mail} ; Qian Ren ; ^{renqian0402@126.com" class="auth_mail}
• 关键词：Eriocheir sinensis ; C-type lectin ; Innate immunity ; Pattern recognition receptor
• 刊名：Fish & Shellfish Immunology
• 出版年：April, 2014
• 年：2014
• 卷：37
• 期：2
• 页码：313-321
• 全文大小：2478 K

文摘

C-type lectin is one of the pattern-recognition proteins of the non-self-innate immune system in invertebrates. In this study, two novel C-type lectin cDNAs (EsCTL1 and EsCTL2) of Eriocheir sinensis were cloned and characterized. EsCTL1 has 169 amino acids, whereas EsCTL2 has 164 amino acids. These two lectins contain one carbohydrate-recognition domain. Phylogenetic analysis showed that EsCTL1 and EsCTL2 were not clustered with other reported lectins from crabs. EsCTL1 and EsCTL2 were expressed only in the hepatopancreas, as detected by real-time PCR. When healthy crabs were challenged with lipopolysaccharide (LPS), peptidoglycan (PGN), Staphylococcus aureus, or Aeromonas hydrophila, the expression levels of EsCTL1 and EsCTL2 were significantly regulated. The recombinant EsCTL1 and EsCTL2 can agglutinate both Gram-positive (S.聽aureus) and Gram-negative bacteria (Vibrio parahaemolyticus and A.聽hydrophila) in a Ca²⁺-dependent manner. The recombinant EsCTL1 and EsCTL2 can directly bind to LPS and PGN and to all tested microorganisms (S.聽aureus, Bacillus thuringiensis, Bacillus subtilis, Escherichia coli, Vibrio natriegens, V.聽parahaemolyticus, and A.聽hydrophila). Furthermore, rEsCTL1 and rEsCTL2 may facilitate the clearance of V.聽parahaemolyticus in聽vivo. These results suggest that EsCTL1 and EsCTL2 may have important roles in the anti-bacterial immunity of Chinese mitten crab.