Oxygen partial pressure modulates 67-kDa laminin receptor expression, leading to altered activity of the green tea polyphenol, EGCG

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• 作者：Shuntaro Tsukamoto ; Shuya Yamashita ; Yoon Hee Kim ; Motofumi Kumazoe ; Yuhui Huang ; Koji Yamada ; Hirofumi Tachibana
• 关键词：EGCG ; (&minus ; )-epigallocatechin-3-O-gallate ; 67LR ; 67-kDa laminin receptor ; HIF-1a ; hypoxia-inducible factor 1 alpha ; MRLC ; myosin II regulatory light chain ; ROS ; reactive oxygen species
• 刊名：FEBS Letters
• 出版年：2012
• 出版时间：21 September, 2012
• 年：2012
• 卷：586
• 期：19
• 页码：3441-3447
• 全文大小：1040 K

文摘

(?)-Epigallocatechin-3-O-gallate (EGCG) exhibits anti-tumor activity mediated via the 67-kDa laminin receptor (67LR). In this study, we found that 67LR protein levels are reduced by exposure to low O₂ levels (5 % ), without affecting the expression of HIF-1¦Á. We also found that EGCG-induced anti-cancer activity is abrogated under low O₂ levels (5 % ) in various cancer cells. Notably, treatment with the proteasome inhibitor, prevented down-regulation of 67LR and restored sensitivity to EGCG under 5 % O₂. In summary, 67LR expression is highly sensitive to O₂ partial pressure, and the activity of EGCG can be regulated in cancer cells by O₂ partial pressure.