Green tea catechins potentiate the neuritogenic action of brain-derived neurotrophic factor: Role of 67-kDa laminin receptor and hydrogen peroxide

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• 作者：Usha Gundimeda^a ; Thomas H. McNeill^a ; Tiffany K. Fan^a ; Ronald Deng^a ; David Rayudu^a ; Zachary Chen^a ; Enrique Cadenas^b ; Rayudu Gopalakrishna^a ; ^{rgopalak@usc.edu" class="auth_mail}
• 关键词：BDNF ; brain-derived neurotrophic factor ; GTPP ; green tea polyphenols ; EGCG ; (&minus ; )-epigallocatechin-3-gallate ; ECG ; (&minus ; )-epicatechin-3-gallate ; EGC ; (&minus ; )-epigallocatechin ; EC ; (&minus ; ) epicatechin ; 67LR ; 67-kDa laminin receptor ; ROS ; reactive oxygen species ; NGF ; nerve growth factor ; NAC ; N-acetyl-l-cysteine ; MTT ; 3-[4 ; 5-dimethylthiazol-2-yl]-2 ; 5-diphenyltetrazolium bromide ; LDH ; lactate dehydrogenase
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：28 February, 2014
• 年：2014
• 卷：445
• 期：1
• 页码：218-224
• 全文大小：1511 K

文摘

Delivery of optimal amounts of brain-derived neurotrophic factor (BDNF) to regions of the brain affected by neurodegenerative diseases is a daunting task. Using natural products with neuroprotective properties, such as green tea polyphenols, would be a highly useful complementary approach for inexpensive long-term treatment of these diseases. In this study, we used PC12(TrkB) cells which ectopically express TrkB, a high affinity receptor for BDNF. They differentiate and induce neurite outgrowth in response to BDNF. Using this model, we show for the first time that treatment with extremely low concentrations (<0.1 渭g/ml) of unfractionated green tea polyphenols (GTPP) and low concentrations (<0.5 渭M) of their active ingredient, epigallocatechin-3-gallate (EGCG), potentiated the neuritogenic ability of a low concentration (2 ng/ml) of BDNF. A synergistic interaction was observed between GTPP constituents, where epigallocatechin and epicatechin, both individually lacking this activity, promoted the action of EGCG. GTPP-induced potentiation of BDNF action required the cell-surface associated 67 kDa laminin receptor (67LR) to which EGCG binds with high affinity. A cell-permeable catalase abolished GTPP/EGCG-induced potentiation of BDNF action, suggesting the possible involvement of H₂O₂ in the potentiation. Consistently, exogenous sublethal concentrations of H₂O₂, added as a bolus dose (5 渭M) or more effectively through a steady-state generation (1 渭M), potentiated BDNF action. Collectively, these results suggest that EGCG, dependent on 67LR and H₂O₂, potentiates the neuritogenic action of BDNF. Intriguingly, this effect requires only submicromolar concentrations of EGCG. This is significant as extremely low concentrations of polyphenols are believed to reach the brain after drinking green tea.