mRNA expression profile of the TNF-α system in LH-induced bovine preovulatory follicles and effects of TNF-α on gene expression, ultrastructure and expansion of cumulus-oocyte complexes cultured in vitro
文摘
The proteins by TNF-α and its receptors (TNFR1 and TNFR2), are present in antral follicles compartments. GnRH treatment increases TNF-α mRNA level in granulosa cells of preovulatory follicles in vivo. In vitro, expression of TNF-α mRNA in cumulus cells is also upregulated by gonadotropins. TNF-α maintains ultrastructure and regulates gene expression during COC culture.