A combination strategy for extraction and isolation of multi-component natural products by systematic two-phase solvent extraction-13C nuclear magnetic resonance pattern recognition and following conical counter-current chromatography separation: Podophyllotoxins and flavonoids from Dysosma versipellis (Hance) as examples
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- 作者：Zhi Yang muyiyuanzhi@126.com" class="auth_mail" title="E-mail the corresponding author ; Youqian Wu 461209323@qq.com" class="auth_mail" title="E-mail the corresponding author ; Shihua Wu ; drwushihua@zju.edu.cn" class="auth_mail" title="E-mail the corresponding author ; drwushihua@hotmail.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：13C NMR-based pattern recognition ; Conical counter-current chromatography ; Dysosma versipellis (Hance) ; Flavonoids ; Natural products isolation ; Podophyllotoxins
- 刊名：Journal of Chromatography A
- 出版年：2016
- 出版时间：29 January 2016
- 年：2016
- 卷：1431
- 期：Complete
- 页码：184-196
- 全文大小：3264 K
文摘
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The HEMWat systems were used systematically for extraction and separation of NPRs.
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13C NMR pattern recognition was used to determine the optimum extraction systems.
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A novel conical CCC device was developed for the purification of Dysosma versipellis.
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Elution-extrusion mode was introduced in the conical CCC purification process.
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17 components were purified by one-step conical CCC separation from HEMWat extracts.